netflow PNH supports extensive PNH educational programme at ESCCA
The 2016 annual meeting of the European Society for Clinical Cell Analysis (ESCCA) delivered an informative overview of cell analysis techniques in Edinburgh, UK, from 11–14 September. Among the activities at ESCCA, there were many opportunities for delegates to learn about the netflow PNH educational programme and attend lectures by key international experts in PNH testing. At the Meet-the-Expert sessions sponsored by Alexion, netflow Steering Committee members were present to provide one-to-one guidance on the practical issues associated with detection of PNH clones by flow cytometry. Four main topics were discussed: PNH testing guidelines for instrument set-up; quality-control programmes; identifying high-risk patient groups for PNH screening; and the interpretation of test results.
Click on the links below to access the PNH-related presentations.a,b
The PNH presentation jointly delivered by Andrea Illingworth (Dahl-Chase Diagnostic Services, Bangor, Maine, USA) and Professor Robert Sutherland (Department of Medicine, University of Toronto, Canada) focused on state-of-the-art protocols for diagnostic flow cytometry for PNH and the requirement for updated consensus guidelines on PNH testing. In a separate lecture, Dr Frank Preijers (Radboud University Medical Centre, Nijmegen, The Netherlands) provided an overview of the life-threatening consequences of PNH and the importance of testing high-risk patients, along with a summary of practical methodology for accurate PNH clone detection.
Additional topics of interest included oral presentations on the national harmonisation of protocols for PNH testing in the UK from Dr Dan Payne (University Hospitals of Leicester NHS Trust, Leicester, UK), the multicentre validation of a flow cytometry protocol for PNH testing from Dr Arianna Gatti (Western Milan Hospital Consortium, Legnano, Italy) and the technical aspects of PNH clone detection from Dr Erik Huys (Radboud University Medical Centre, Nijmegen, the Netherlands).
PNH poster presentationsa,b
- “A single-center experience of flow cytometry for paroxysmal nocturnal hemoglobinuria” (Ahmad Ahmadi et al; poster 04) presented epidemiological results from a 6-year Swedish study on PNH clone detection.
- “Dynamics of a PNH-clone in aplastic anemia patients during immunosuppressive therapy” (Zalina Fidarova et al; poster 07) described results from long-term monitoring of PNH clone size in a Russian cohort of patients with aplastic anaemia, demonstrating variation in initial clone size and long-term clone development between these patients. Download poster
- “5 years PNH screening results in Russian patients groups” (Elena Babenko et al; poster 23) emphasised the requirement for continual monitoring of PNH clone size in patients from a 5-year study in Russia. Download poster
- “Multicentric evaluation of the current medical indication for paroxysmal nocturnal hemoglobinuria diagnostic screening” (Martin Perez-Andres et al; poster 30) presented work on the high efficiency of PNH-clone detection in a large-scale Spanish study, based on the screening recommendations from the 2010 consensus guidelines on PNH diagnostic testing. Download poster
- “PNH reticulocytes identification as a fast screening method for PNH-clone evaluation in patients” (Elena Naumova et al; poster 48) presented results from a large Russian study highlighting the requirement for a standardised method of PNH-clone detection given the low prevalence of PNH and the relative lack of PNH-positive patient samples. Download poster