Peripheral blood samples are recommended for evaluating both red blood cells and white blood cells. Testing should be performed on at least two different cell populations (not lymphocytes). Bone marrow samples are not appropriate outside the research setting as immature myeloid populations may express lower levels of glycosylphosphatidylinositol (GPI)-anchored proteins, which complicates interpretation.
Peripheral blood is the preferred specimen for paroxysmal nocturnal haemoglobinuria (PNH) testing by flow cytometry1,2
Recommendations for PNH sample handling
Recommendations on the source, storage and handling of samples for PNH testing1
|Anticoagulant||EDTA or heparin (no data are available to indicate a preferred agent)|
|Volume||Minimum 1 mL; 3 mL is adequate for most testing, though more may be needed if WBC count is very low|
|Maximum sample age||≤7 days for RBCs; <48 h for WBCs|
|Sample storage||4°C after 24 h|
|Cell populations evaluated||
EDTA, ethylenediaminetetraacetic acid; PNH, paroxysmal nocturnal haemoglobinuria; RBC, red blood cell; WBC, white blood cell
- Borowitz MJ et al. Cytometry B Clin Cytom 2010; 78B: 211-230.
- Sutherland DR et al. Cytometry B Clin Cytom 2012; 82B: 195-208.